Designing and evaluation of Dot-ELISA for diagnosis of Fasciola infection in cattle
نویسندگان
چکیده
Fasciolosis is a disease caused by liver fluck of the genus of Fasciola. Diagnosis of fasciolosis has been challenging for a long period due to low sensitivity of the coprological diagnostic method. In this study, an in-house Dot-ELISA method; using excretion-secretory (ES Ag) and Crude (Cr Ag) antigens of Fasciola was described for diagnosis of fasciolosis in cattle. For this purpose, the sera specimens of slaughtered cattle were taken and examined for Fasciola infection. Sera from two groups of cattle, one infected with Fasciola (n = 60) and the other non-infected with Fasciola (n = 60), were used in the Dot- ELISA test. All sera were tested and evaluated. Except specificity, other parameters such as, sensitivity, accuracy, positive and negative predictive values of Dot- ELISA with ES Ag were better than those of Dot- ELISA with Cr Ag. In conclusion, excretory-secretory antigen dependent Dot-ELISA can be used as a reliable sero-diagnostic test for Fasciola infection in cattle.
منابع مشابه
Designing and evaluation of Dot-ELISA for diagnosis of Fasciola infection in cattle
Fasciolosis is a disease caused by liver fluck of the genus of fasciola. Diagnosis of fasciolosis has been challenging for a long period due to low sensitivity of the coprological diagnostic method. In this study, an in-house Dot-ELISA method; using excretion–secretory (ES Ag) and Crude (Cr Ag) antigens of fasciola was described for diagnosis of fasciolosis in cattle. For this...
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Fasciolosis is a zoonotic parasitic disease with worldwide distribution. The disease causes significant economic losses in herd industry including decrease animal products such as meat, milk and infertility reduction. Human can acquire infection as accidental host especially in areas with high level of raining and humidity. Previous studies showed the prevalence of infection among meat-product ...
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Aim The objective of the present study was to know the seroprevalence status of Fasciola gigantica infection in cattle and buffaloes using cysteine proteinase (CP) antigen in dot enzyme-linked immunosorbent assay (ELISA) format under field conditions. Materials and Methods As per the standard protocol, the sera were collected from the blood of 112 cattle and 38 buffaloes of coastal areas of N...
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